Terpenes based hydrophobic deep eutectic solvents for dispersive liquid-liquid microextraction of aliphatic aldehydes in drinking water and alcoholic beverages.

Aliphatic aldehydes are a class of organic compounds containing aldehyde groups, which are widespread, and closely related to people's daily life and health. In this work, a series of terpenes based hydrophobic deep eutectic solvents were designed and synthesized using hexafluoroisopropanol as hydrogen bond donor and menthol/thymol as hydrogen bond acceptor. Then they are used as extraction solvent in dispersive liquid-liquid microextraction for extracting and determining seven aliphatic aldehydes from drinking water and alcoholic beverage combined with high performance liquid chromatography-ultraviolet. Due to the fact that these hydrophobic deep eutectic solvents are liquid at the room temperature, a density greater than that of water, a lower viscosity (≤26.10 mPa s, 25 °C), after extraction and centrifugation, the microvolume DES-rich phase in the bottom is convenient for collection and direct analysis without further dissolution or dilution with organic solvents. Some factors affecting the extraction recovery were optimized by one-variable-at-a-time and response surface methodology. Under the optimal conditions, the enrichment factors for the seven aliphatic aldehydes were 48-56. The method had good performance: linear ranges of 1.0-200, 0.5-200, 0.2-200, 0.4-400, 1.0-400, 0.4-400 and 0.4-400 µg L-1 for seven aliphatic aldehydes (r2 ≥ 0.9949), limits of detection of 0.1-0.5 µg L-1, intra-day and inter-day precisions <4.9%. The recoveries of seven aliphatic aldehydes ranged from 76.0 to 119.0%. The proposed dispersive liquid-liquid microextraction method is simple, rapid, highly efficient, and green, which effectively reduces the amount of toxic chemical reagents used and their impact on the environment. Rapid and efficient detection of aliphatic aldehydes helps ensure a healthy diet and has great application prospects in food safety analysis.

A novel fluorescent aptasensor for sensitive and selective detection of environmental toxins fumonisin B1 based on enzyme-assisted dual recycling amplification and 2D δ-FeOOH-NH2 nanosheets.

Fumonisin (FB) is a pervasive hazardous substance in the environment, presenting significant threats to human health and ecological systems. Thus, the selective and sensitive detection of fumonisin B1 (FB1) is crucial due to its high toxicity and wide distribution in corn, oats, and related products. In this work, we developed a novel and versatile fluorescent aptasensor by combining enzyme-assisted dual recycling amplification with 2D δ-FeOOH-NH2 nanosheets for the determination of FB1. The established CRISPR/Cas12a system was activated by using activator DNA (aDNA), which was released via a T7 exonuclease-assisted recycling reaction. Additionally, the activated Cas12a protein was utilized for non-specifically cleavage of the FAM-labeled single-stranded DNA (ssDNA-FAM) anchored on δ-FeOOH-NH2 nanosheets. The pre-quenched fluorescence signal was restored due to the desorption of the cleaved ssDNA-FAM. Due to the utilization of this T7 exonuclease-Cas12a-δ-FeOOH-NH2 aptasensor for signal amplification, the detection range of FB1 was expanded from 1 pg/mL to 100 ng/mL, with a limit of detection (LOD) as low as 0.45 pg/mL. This study not only provides novel insights into the development of fluorescence biosensors based on 2D nanomaterials combined with CRISPR/Cas12a, but also exhibits remarkable applicability in detecting other significant targets.

Acid induced dispersive liquid-liquid microextraction based on in situ formation of hydrophobic deep eutectic solvents for the extraction of bisphenol A and alkylphenols in water and beverage samples.

This study describes the development of an acid induced dispersive liquid-liquid microextraction method based on the in situ formation of hydrophobic deep eutectic solvents for the extraction of bisphenol A and alkylphenols from environmental water and beverage samples. Hydrochloric acid altered the hydrophilic-hydrophobic state of fatty acid salts to obtain hydrophobic fatty acids, which formed hydrophobic deep eutectic solvents with analytes in situ to extract the analytes. Under optimized conditions, the limits of detection and limits of quantitation were 0.03-0.1 µg L-1 and 0.12-0.3 µg L-1, the intraday and interday relative standard deviations were less than 3.9 %, and the enrichment factor was 29-32. The recoveries of bisphenol A and alkylphenols were 95.9-104.9 % and 86.9-105.0 %, respectively. The extraction process used only hydrochloric acid and fatty acid salts, and the extraction process required less than 1 min. This method has the advantages of simplicity, speed, low cost and environmental friendliness.

Constructing an eco-friendly and ratiometric fluorescent sensor for highly efficient detection of mercury ion in environmental samples.

Mercury ion (Hg2+) is a highly toxic and ubiquitous pollutant, whose effective detection has aroused widespread concern. A novel ratiometric fluorescent sensor has been designed to rapidly and efficiently detect Hg2+ based on blue/red carbon dots (CDs) with environmental friendliness. This sensor was well characterized via TEM, FTIR, XPS, UV-vis, and zeta potential analysis and displayed excellent fluorescence properties and stability. The fluorescence of blue CDs at 447 nm was significantly quenched with the addition of Hg2+ resulted from the static quenching, whereas that of red CDs at 650 nm remained invariable. A sensitive method for Hg2+ determination was constructed in the range of 0.05-7.0 nmol mL-1 with optimal conditions, and the detection limit was down to 0.028 nmol mL-1. Meanwhile, compared to other 17 metal ions, the ratiometric fluorescent sensor exhibited high selectivity for Hg2+. Furthermore, satisfied recoveries had also been obtained for measuring trace Hg2+ in practical environmental samples. This developed ratiometric fluorescent sensor provided a reliable, environmental-friendly, rapid, and efficient platform for the detection of Hg2+ in environmental applications.

A novel carbonized polymer dots-based molecularly imprinted polymer with superior affinity and selectivity for oxytetracycline removal.

Molecularly imprinted polymers (MIPs) synthesized from chain functional monomers are restricted by spatial extension and exhibit relatively poor affinity and selectivity; this results in unsatisfactory applications in complex media. In this study, we prepared unique spherical carbonized polymer dots (CPDs-OH) via the incomplete carbonization of 1-allyl-3-vinylimidazolium bromide and ethylene glycol, and used it as a functional monomer to prepare a newly imprinted polymer (CPDs-OH@MIP) in aqueous media. As a result, the CPDs-OH@MIP exhibited effective recognition of oxytetracycline with an impressive imprinting factor of 6.17, surpassing MIPs prepared with chain functional monomers (1-3). Furthermore, CPDs-OH@MIP exhibited excellent adsorption for oxytetracycline (278.52 mg g-1) and achieved equilibrium in 30 min, with stronger resistance to coexisting cations, anions, and humic acid. Compared to other MIPs and adsorbents, the recognition performance of CPDs-OH@MIP improved 2-4 times; this polymer could remove >92.1% of oxytetracycline in real water samples with at least 10 cycle times. CPDs-OH@MIP prepared using the special spherical monomer forms a denser structure with fewer nonimprinted regions and precisely imprinted sites, remarkably improving the affinity and selectivity of MIPs combined via hydrogen bonds and electrostatic and π-π interactions. Our proposed strategy provides an effective basis for breakthroughs in the practical application of MIPs.

[Effects of pH, Calcium, and Phosphate on the Solubility of Arsenic in Paddy Soil Based on Surface Complexation Modeling].

This study aimed to obtain a better understanding on the environmental behavior of As in paddy soil and to reveal the influence mechanisms of different environmental factors on the availability of As in the soil solution. The effects of pH, calcium, and phosphate on the solubility and speciation distribution of As in the paddy soil collected from Zhuzhou of Hunan province were studied by combining the adsorption experiments with the NOM-CD model. The results showed that the minimum concentration of soluble As in the soil was at approximately a pH of 6.0, which was mainly affected by both electrical interactions and site competition between Ca2+, PO43-, As(Ⅲ), and As(Ⅴ). The adsorption of As onto soil particles could be increased by an increase in Ca2+ in the soil system, leading to the decrease in soluble As concentration. This effect became significant at a higher pH, because adsorbed Ca2+ increased the positive charge on (hydr)oxide surfaces. With phosphate addition, the reduction in As(Ⅴ) in the soil was inhibited at pH<5.5, whereas it was promoted at pH>5.5. Moreover, the concentration of soluble As(Ⅲ) and As(Ⅴ) in the soil solution was dramatically increased with the addition of phosphate owing to the competitive adsorption between As and phosphate. At a lower background of Ca2+, there was a higher fraction of As(Ⅲ) in the soil either with or without phosphate addition. This phenomenon might be caused by the higher bioavailability of phosphorus in soil at a lower concentration of Ca2+, which favors the dissimilatory reduction of As or iron (hydr)oxides. The results indicated that the NOM-CD model could predict the influence of pH, calcium, and phosphate on the solubility and speciation distribution of As in paddy soil and reveal its main mechanisms. Therefore, the NOM-CD model would provide the quantitative and scientific method for evaluating the risk of As in soils or other solid-water systems.

Switchable ROS Scavenger/Generator for MRI-Guided Anti-Inflammation and Anti-Tumor Therapy with Enhanced Therapeutic Efficacy and Reduced Side Effects.

Photosensitizer in photodynamic therapy (PDT)  accumulates in both tumor and adjacent normal tissue due to low selective biodistribution, results in undesirable side effect with limited clinic application. Herein, an intelligent nanoplatform is reported that selectively acts as reactive oxygen species (ROS) scavenger in normal tissue but as ROS generator in tumor microenvironment (TME) to differentially control ROS level in tumor and surrounding normal tissue during PDT. By down-regulating the produced ROS with dampened cytokine wave in normal tissue after PDT, the nanoplatform reduces the inflammatory response of normal tissue in PDT, minimizing the side effect and tumor metastasis in PDT. Alternatively, the nanoplatform switches from ROS scavenger to generator through the glutathione (GSH) responsive degradation in TME, which effectively improves the PDT efficacy with reduced GSH level and amplified oxidative stress in tumor. Simultaneously, the released Mn ions provide real-time and in situ signal change of magnetic resonance imaging (MRI) to monitor the reversal process of catalysis activity and achieve accurate tumor diagnosis. This TME-responsive ROS scavenger/generator with activable MRI contrast may provide a new dimension for design of next-generation PDT agents with precise diagnosis, high therapeutic efficacy, and low side effect.

Moisture-resistant and green cyclodextrin metal-organic framework nanozyme based on cross-linkage for visible detection of cellular hydrogen peroxide.

A moisture-resistant and green cyclodextrin metal-organic framework (CD-MOF) nanosheet has been prepared via an one-pot antisolvent synthesis procedure. After the treatment of in situ chemical cross-linkage, the two-dimensional (2D) cross-linked CD-MOF exhibited both peroxidase (POD) and oxidase (OXD) enzymatic activities, as well as hydrolytic stability. On the basis of its POD mimics function, the proof-of-concept biosensors were constructed to realize the colorimetric detection for H2O2 and glucose, respectively. In vitro cytotoxicity experiments showed that the 2D cross-linked CD-MOF nanozymes still maintained excellent biocompatibility even at a concentration reaching up to several mg/mL. The in situ colorimetric detection of H2O2 secreted by HepG2 cells further confirmed its promising biocompatibility, showing its great promises as label-free colorimetric probe in early cancer detection and pathological process monitoring.

Fast and efficient "on-off-on" fluorescent sensor from N-doped carbon dots for detection of mercury and iodine ions in environmental water.

A kind of nitrogen doped carbon dots (N-CDs) was facilely fabricated from polyethyleneimine and anhydrous citric acid, and which was adopted to develop a neoteric "on-off" and "off-on" fluorescent sensor for rapidly and efficiently sensing Hg2+ and I-. The fluorescence of N-CDs was notably quenched (off) in the existence of Hg2+ derived from strong interaction and the electron transfer between N-CDs and Hg2+, while the quenched fluorescence of the N-CDs and Hg2+ system was strikingly regained by addition of I- (on) resulted from the separation of N-CDs and Hg2+ due to the higher binding preference between Hg2+ and I-. Under optimal conditions, the linear detection ranges were 0.01-20 µM for Hg2+ and 0.025-7 µM for I-, respectively. Meanwhile, the detection limits could be down to 3.3 nM for Hg2+ and 8.5 nM for I-, respectively. Satisfied recoveries had also been gained for measuring Hg2+ and I- in practical water samples. The constructed "on-off-on" fluorescent sensor provided a simple, rapid, robust and reliable platform for detecting Hg2+ and I- in environmental applications.

One-step derivatization and temperature-controlled vortex-assisted liquid-liquid microextraction based on the solidification of floating deep eutectic solvents coupled to UV-Vis spectrophotometry for the rapid determination of total iron in water and food samples.

In our work, hydrophobic thymol-based deep eutectic solvents (DESs) with strong reducibility, a lower density than water, and a slightly higher melting point than room temperature were synthesized. Based on these solid DESs, one-step derivatization and temperature-controlled vortex-assisted liquid-liquid microextraction based on the solidification of a floating DES (TC-VA-LLME-SFDES) via UV-Vis spectrophotometry for the rapid determination of total iron was developed. The derivatization and TC-VA-LLME were carried out simultaneously by the addition of DES as both the reducing agent and extraction solvent. After optimization, the calibration curve (5-250 µg L-1, R2 = 0.9982), limit of detection (1.5 µg L-1), limit of quantitation (5.0 µg L-1), precision (≤4.0%) and enrichment factor (92) was obtained. This method was applied for the determination of total iron in water and food samples with satisfactory recoveries (85.4-106.2%). One-step derivatization and TC-VA-LLME only required 2 min. Furthermore, this method opened up the application of solid DESs in liquid-liquid microextraction.

Surface imprinted polymer on a metal-organic framework for rapid and highly selective adsorption of sulfamethoxazole in environmental samples.

The demand for the removal of pollutants in aqueous solution has triggered extensive studies to optimize the performance of adsorbents, but the adsorption rate and selectivity of adsorbents have been overlooked. Hierarchically ordered porous vinyl-functionalized UIO-66 was used as supporter to prepare a surface molecular imprinted polymer (MIP-IL@UIO-66). The UIO-66 with large specific surface area significantly increased the number of active site of polymer, and so the MIP-IL@UIO-66 can achieve the rapid and highly selective adsorption of sulfamethoxazole (SMZ) in water. The structure and morphology of MIP-IL@UIO-66 was examined using scanning electron microscopy, Fourier transform infrared spectroscopy, nitrogen adsorption-desorption isotherms, thermogravimetry, X-ray photoelectron spectroscopy, and X-ray powder diffraction. Results indicate that the presented MIP-IL@UIO-66 has an ultrafast equilibrium rate (equilibrium time, 10 min), large adsorption capability (maximum capacity, 284.66 mg g-1), excellent adsorption selectivity (selectivity coefficient, 11.36), and good reusability (number of cycles, 5 times) via equilibrium adsorption experiments. Subsequently, as a novel solid phase extraction (SPE) adsorbent, the adsorption performance of SMZ onto MIP-IL@UIO-66 was better than that of a commercial SPE adsorbent. A MISPE column combined with high-performance liquid chromatography (HPLC) was presented to detect SMZ in water, soil, egg, and pork samples with recoveries of 91-106%. Hydrogen bonds, electrostatic and π-π interactions, and molecular memory were attributed to recognizing the SMZ of MIP-IL@UIO-66.

Novel recyclable acidic hydrophobic deep eutectic solvents for highly efficient extraction of calcium dobesilate in water and urine samples.

Deep eutectic solvents (DESs) have been considered to be one of the most promising green alternatives of conventional volatile solvents for liquid-liquid separation. However, acidic hydrophobic DESs have been less studied although they are of great importance for the extraction of compounds which are unstable in alkaline conditions. In this study, a novel family of acidic hydrophobic deep eutectic solvents was designed and prepared from methyl trioctyl ammonium chloride and a series of haloacetic acids. For the first time, the obtained DESs were used for extraction and determination of calcium dobesilate, which is an extensively used medicine for treating vascular diseases, but it can be easily oxidized under alkaline and neutral conditions. The factors influencing extraction process including pH, temperature, extract time, inorganic salts and organic coexistence were systematically investigated. It is found that these DESs exhibited outstanding extraction performance towards calcium dobesilate. The extraction equilibrium time was only 3 min in a wide range of pH (1.2-9.2) at room temperature and the extraction capacity was up to 504 mg/g. The detection limit of calcium dobesilate extracting from water samples was 0.05 µg/L and the limit of quantification was 0.5 µg/L. A variety of inorganic salts with the concentration up to 1.0 mol/L and common coexisting organic compounds, such as glucose and starch, with the concentration more than 1000 times higher than the target analyte had no obvious impact on the extraction efficiency. The relative recovery for real samples ranged from 93.2% to 108.6%. Furthermore, the DESs could be recycled and regenerated through back extraction. After fifteen cycles, the extraction efficiency was still up to 99%. Finally, the extraction and back extraction mechanism was discussed in detail.

Green preparation of carbon dots from Momordica charantia L. for rapid and effective sensing of p-aminoazobenzene in environmental samples.

p-Aminoazobenzene (pAAB) is a hazardous azo dye that causes considerable harm to human health and the environment. The development of novel and sensitive sensors for the rapid detection of pAAB is in high demand. In this study, a simple fluorescent sensor for pAAB detection is designed based on carbon dots (CDs) which are prepared using green carbon source of Momordica charantia L. via a facile hydrothermal approach. The fluorescence spectra of CDs exhibit considerable overlap with the absorption band of pAAB, and the fluorescence is specifically suppressed in the presence of pAAB ascribed to the inner filter effect. Good and wide linearity is observed in the pAAB concentration range of 0.01-12.5 µg mL-1 with a lower detection limit of 3.9 ng mL-1. The established method achieves good results with a rapid analysis of pAAB in different practical water and soil samples. The as-constructed fluorescent sensor provides a simple, rapid, economical and eco-friendly platform and possesses prospective applications for the effective, selective and sensitive detection of pAAB in the environmental field.

Comparative evaluation of biochar, pelelith, and garbage enzyme on nitrogenase and nitrogen-fixing bacteria during the composting of sewage sludge.

This study investigated the effects of garbage enzyme (GE), pelelith (PL), and biochar (BC) on nitrogen (N) conservation, nitrogenase (Nase) and N-fixing bacteria during the composting of sewage sludge. Results showed that the addition of GE, PL, and BC reduced NH3 emissions by 40.9%, 29.3%, and 67.4%, and increased the NO3-N contents of the end compost by 161.4, 88.2, and 105.8% relative to control, respectively, thus increasing the TN content. Three additives improved Nase, cellulase, and fluorescein diacetate hydrolase (FDA) activities and the abundances of nifH gene, and the largest increase was BC, followed by PL and GE. In addition, the additives also markedly influenced the succession of N-fixing bacteria, and significantly increased the abundance of Proteobacteria during the whole process. The BC and PL additions strengthened the sensitivity of N-fixing bacteria to environmental variables, and FDA, TN, moisture content, and NO3-N significantly affected the N-fixing bacteria at genus level.

Chemical characteristics and source apportionment of PM2.5 in a petrochemical city: Implications for primary and secondary carbonaceous component.

To study the pollution features and underlying mechanism of PM2.5 in Luoyang, a typical developing urban site in the central plain of China, 303 PM2.5 samples were collected from April 16 to December 29, 2015 to analyze the elements, water soluble inorganic ions, organic carbon and elemental carbon. The annual mean concentration of PM2.5 was 142.3 µg/m3, and 75% of the daily PM2.5 concentrations exceeded the 75 µg/m3. The secondary inorganic ions, organic matter and mineral dust were the most abundant species, accounting for 39.6%, 19.2% and 9.3% of the total mass concentration, respectively. But the major chemical components showed clear seasonal dependence. SO42- was most abundant specie in spring and summer, which related to intensive photochemical reaction under high O3 concentration. In contrast, the secondary organic carbon and ammonium while primary organic carbon and ammonium significantly contributed to haze formation in autumn and winter, respectively. This indicated that the collaboration effect of secondary inorganic aerosols and carbonaceous matters result in heavy haze in autumn and winter. Six main sources were identified by positive matrix factorization model: industrial emission, combustion sources, traffic emission, mineral dust, oil combustion and secondary sulfate, with the annual contribution of 24%, 20%, 24%, 4%, 5% and 23%, respectively. The potential source contribution function analysis pointed that the contribution of the local and short-range regional transportation had significant impact. This result highlighted that local primary carbonaceous and precursor of secondary carbonaceous mitigation would be key to reduce PM2.5 and O3 during heavy haze episodes in winter and autumn.

Garbage enzymes effectively regulated the succession of enzymatic activities and the bacterial community during sewage sludge composting.

This study evaluated nitrogen transformation, enzymatic activities and bacterial succession during sewage sludge composting with and without garbage enzymes (GE and CK, respectively). The results showed that GE addition significantly increased fluorescein diacetate hydrolase (FDA), cellulase, and nitrogenase activities during the composting process. GE addition reduced the cumulative NH3 emissions by 66.5%, increased the peak NH4-N content by 26.3% and increased the total nitrogen (TN) content of the end compost by 39.2% compared to CK. Microbiological analysis revealed that GE addition significantly increased the relative abundance of Firmicutes during the thermophilic and cooling phases relative to CK. The selected factors affected the bacterial community composition in the following order: NH4-N > TOC > FDA > TN > C/N. Network analysis also showed that the enzymes were secreted mainly by Bacillus and norank_f_Caldilineaceae in GE, while they were secreted primarily by norank_f_Methylococcaceae in CK during the composting process.

Air-assisted liquid-liquid microextraction based on the solidification of floating deep eutectic solvents for the simultaneous determination of bisphenols and polycyclic aromatic hydrocarbons in tea infusions via HPLC.

Deep eutectic solvents (DESs) are a new class of green "designer solvent"; its physicochemical properties can be easily tuned by adjusting DES' constituents, chemical ratio and water content. In this study, three hydrophobic DESs with low viscosity, low density, and melting points close to room temperature were designed and synthesized. Based on these DESs, an air-assisted liquid-liquid microextraction technique was developed based on the solidification of floating DESs for the simultaneous determination of bisphenols and polycyclic aromatic hydrocarbons (PAHs) via HPLC. The microextraction parameters were optimized via the Plackett-Burman design and response surface methodologies. The method shows satisfactory linearity (R2 ≥ 0.9928), a low limit of detection (0.16-0.75 µg L-1) and satisfactory precision (≤2.3%), and was successfully applied for the simultaneous determination of bisphenols and PAHs from tea infusions with satisfactory recoveries (82.0-116.6%). This method is simple, rapid, economical, environmentally compatible, dispersive solvent-frees and centrifugation-free, and has promising applications in food safety.

A highly sensitive and selective method for the determination of ceftiofur sodium in milk and animal-origin food based on molecularly imprinted solid-phase extraction coupled with HPLC-UV.

The effective analysis of cephalosporin antibiotics in food animals has attracted considerable attention. Herein, a high-performance liquid chromatograph equipped with a UV method based on molecularly imprinted-solid phase extraction (MISPE-HPLC-UV) was developed for preconcentration, cleanup and determination of ceftiofur sodium (CTFS) in food samples. In this method, an eco-friendly molecularly imprinted polymer (MIP) was synthesized and employed as an adsorbent, which exhibited excellent selectivity towards CTFS in water, and adsorption equilibrium could be reached within 1 h. Under the optimized conditions, good linearity was obtained for CTFS in the range of 0.005-1.0 mg L-1 with a lower LOD of 0.0015 mg L-1, and the average recoveries were higher than 91.9% (RSD less than 8.5%) at three spiked levels in milk, chicken, pork and beef samples. After 20 cycles, the recovery of the MISPE cartridge for CTFS was still higher than 95%, which proved that the MISPE-HPLC-UV method was highly sensitive and selective for the analysis of CTFS in food samples.

Eco-friendly ionic liquid imprinted polymer based on a green synthesis strategy for highly selective adsorption tylosin in animal muscle samples.

A novel eco-friendly molecularly imprinted polymer (MIP) was proposed as solid-phase extraction (SPE) adsorbent to selective adsorption tylosin (TYL) in animal muscle samples. The MIP was synthesized in aqueous by using 1,4-butanediyl-3,3-bis-1-vinyl imidazolium chloride and 2-acrylamide-2-methylpropanesulfonic acid as bifunctional monomer. The obtained MIP had excellent selectivity towards TYL in water, and the maximum binding capacity can reach 123.45 mg g-1. Combined with high-performance liquid chromatography, the presented MIP can be used as SPE sorbent to recognize and detect TYL in the range of 0.008 to 0.6 mg L-1 (R2 = 0.9995). The limit of detection and limit of quantification were 0.003 mg L-1 and 0.008 mg L-1, and the intraday and interday precision were 1.05% and 3.36%, respectively. Under the optimal condition, the established MIP-SPE-HPLC method was successfully applied to separate and determine trace TYL in chicken, pork, and beef samples with satisfactory recoveries ranged from 94.0 to 106.3%, and the MIP-SPE cartridge can be cycled at least 20 times. This study implies a promising green MIP-SPE-HPLC method for highly selective adsorption and analysis trace TYL in complex matrices.

Effects of urease inhibitors on enzymatic activities and fungal communities during the biosolids composting.

This study evaluated the influences of urease inhibitors (UIs) on nitrogen conversion, enzyme activities, and fungal communities during aerobic composting. Results showed that UI addition reduced NH3 emissions by 22.2% and 21.5% and increased the total nitrogen (TN) content by 9.7% and 14.3% for the U1 (0.5% UI of the dry weight of the mixture) and U2 (1% UI of the dry weight of the mixture) treatments, respectively. The addition of UI inhibited the enzyme activity during thermophilic stage while increased enzyme activity during the cool and maturity stages. Ascomycota, Basidiomycota and unclassified fungi were the main phyla, and Ascomycota increased significantly during the maturity period. Network analysis showed that Aspergillus, Penicillium, Trichoderma, Talaromyces, Peseudeurotium, and Exophiala were the main "connecting" genera. The redundancy analysis (RDA) showed that the fungal community was mainly influenced by temperature, DOC, pH, and urease. The results suggested that UI was an effective additive for nitrogen conservation and the increase of enzyme activity reduce nitrogen loss and promote enzyme activity during biosolids composting.